Steps To Troubleshoot Wax Cutting Issues For Troubleshooting

Feed is not formed

Sections are looped, skipped, or inserted into the block; Ribbon slightly different

• the wax is too hard; should be automatically more sticky.
• Space / block is too cold / hot
• Sections are too thick
• Can’t display ribbon individually
• The raised angle of the knife is often too small
• The blade may crust on a dirty surface
• Something in the microtome is damaged. loose

• Select a wax with a lower melting point
• Adjust the preset setting

< li> Decrease micrometer.

• Slightly dull blade; Notch on one edge
• Increase the blade clearance angle from 3 to 8 degrees.
• Replace the blade with a good one.
• Tighten all pliers

Inclined belts

Straight belts, difficult to get straight shape

• Top and bottom edges of the block are not parallel to the edge of the blade / Sides of the block are not perpendicular to the blade

• Uneven surface of the block; At least one edge of the block is rough in front of the others

• Adjust the block holder so the edges of the block are parallel to the knife

< li> The branded block must be oriented so that the top and bottom of the block are parallel to the blade and the sides of the block are vertical to the blade.

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Squeeze and crease the stripes

No smooth stripes

• Block closed while hot
• Slow and irregular rotations increase compression
• Paraffin is too sticky; should be heavier
• dull knife
• knife distance is considered too small

• Block should be well cooled
• To achieve uniform twisting, use constant wheel rotation.
• If you need paraffin wax because of a higher melting point
• Install a premium blade.
• Increase knife spacing

Large holes, no tissue or soft cuts

Pieces of tissue are not visible on the microscope slides

• The tissue is not tamped flat; does not appear when cut
• The fabric is not properly processed and does not form a cut (especially if the rotation is rough)
• A small part of the fabric tears on contact with warm water < br>
• Block because it is very aggressive

• Depending on the problem / symptom, re-insert or paste as needed cell after revision

Tapes attaches to microtome or children

Ribbon is tricky because you can cut everything

• Static electricity

< / ul>

• Use an anti-static spray; cut into smaller strips for better control; To avoid static charges, DO NOT wipe the microtome persistently and / or vigorously, especially with ParaPel ^®

The blade cuts into the block; Solar cells may be lost

• The fabric in the matrix is ​​not fixed
• The unit is not correctly inserted into the cassette

< li> Knife / other microtome tip not stuck

• Block not secure in block holder
• Aggressive scheduled processing with fast rotation

< ul>

• Re-seal the fabric
• Reattach the unit to secure the cassette correctly
• Pull on everything clamps to make sure all parts are properly secured.
• Anchor the block to the support Avert.
• Drive slower